Limma, which stands for "Linear Models for Microarray Data," is a statistical software package widely used in the field of bioinformatics and computational biology for the analysis of gene expression data. Originally developed for microarray data, it has been extended to handle various types of high-throughput data, including RNA-Seq. Limma is primarily used to identify differentially expressed genes (DEGs) by applying linear models and empirical Bayes methods.
