While bisulfite sequencing is a powerful tool, it has some limitations:
DNA Degradation: The bisulfite treatment process can degrade DNA, affecting the quality and quantity of the material available for sequencing. Incomplete Conversion: Incomplete conversion of unmethylated cytosines to uracil can lead to false positives in methylation detection. Cost and Time: Bisulfite sequencing can be costly and time-consuming, especially when analyzing large genomes or multiple samples.
