While Excavator2 is a powerful tool, it does have some limitations:
Data Quality: The accuracy of CNA detection depends on the quality of the sequencing data. Low-quality data can lead to false positives or negatives. Computational Resources: The tool requires significant computational resources, which may be a barrier for some research labs. Complexity: The tool's complexity may require specialized knowledge in bioinformatics and genomics for proper use and interpretation of results.