Despite its widespread use, hematoxylin staining has some limitations:
Lack of Specificity: While it provides excellent nuclear detail, hematoxylin does not distinguish between different types of cancer cells. Subjectivity: Interpretation of H&E-stained slides can be subjective and relies heavily on the expertise of the pathologist. Supplementary Techniques Needed: Often, additional techniques like immunohistochemistry or molecular diagnostics are required for a definitive diagnosis.