While MSI testing is a powerful tool, it has certain limitations:
1. False-Negative Results - MSI testing may yield false-negative results if the tumor does not have enough microsatellite regions analyzed or if the tissue sample is inadequate.
2. Interpretation Challenges - The interpretation of MSI testing results can sometimes be complex, particularly with regard to low-level MSI (MSI-L) where the clinical significance is less clear.
3. Cost and Accessibility - MSI testing, particularly using advanced methods like NGS, can be costly and may not be readily available in all healthcare settings.
