What are the Limitations of qPCR in Cancer Studies?
While qPCR is a powerful tool, it has some limitations. One major limitation is that qPCR requires prior knowledge of the target sequence, meaning it can only detect known mutations and gene expressions. Additionally, qPCR can be affected by the quality and quantity of the starting material, which can lead to variability in results. Finally, qPCR is not suitable for detecting large genomic changes, such as chromosomal rearrangements or large deletions.
