qPCR data is analyzed by measuring the threshold cycle (Ct) value, which is the point at which the fluorescence signal exceeds the background level. The Ct value is inversely proportional to the amount of target nucleic acid in the sample. By comparing Ct values between different samples, researchers can quantify the relative expression levels of target genes. Normalization against housekeeping genes is often used to account for variations in starting material and experimental conditions.
