While WES is a powerful tool, it has several limitations: 1. Non-Coding Regions: WES does not capture mutations in non-coding regions of the genome, which can also play a role in cancer. 2. Structural Variants: WES may miss large structural variants and copy number variations that are detected more easily with WGS. 3. Coverage Gaps: Some exonic regions may not be well-covered due to technical limitations, potentially missing important mutations.
