What is the Process of Conducting WES in Cancer Studies?
The process of conducting WES in cancer studies involves several steps: 1. Sample Collection: Tumor and matched normal tissues are collected from the patient. 2. DNA Extraction: DNA is extracted from the collected tissues. 3. Library Preparation: The DNA is fragmented, and libraries are prepared for sequencing. 4. Exome Capture: Exonic regions are captured using probes designed to target all known exons. 5. Sequencing: The captured exonic regions are sequenced using high-throughput sequencing technologies. 6. Data Analysis: Sequence data is analyzed to identify mutations, which are then validated through additional experiments.
