What are the Technologies Used in Multiplex Imaging?
Several technologies are employed in multiplex imaging, including:
1. Immunohistochemistry (IHC): This technique uses antibodies and colorimetric detection to visualize protein expression in tissues. 2. Immunofluorescence (IF): Similar to IHC but uses fluorescent dyes for higher sensitivity and multiplexing capability. 3. Mass Cytometry (CyTOF): Combines flow cytometry and mass spectrometry to analyze multiple parameters simultaneously. 4. Multiplexed Ion Beam Imaging (MIBI): Utilizes secondary ion mass spectrometry for high-dimensional imaging of tissue sections. 5. Digital Pathology: Incorporates whole-slide imaging and computational analysis for quantitative assessment of tissue samples.
