What are the Types of PCR Techniques Used in Cancer Research?
Several types of PCR techniques are utilized in cancer research, including:
Conventional PCR: This is the basic form of PCR, used to amplify DNA sequences for further analysis. Real-Time PCR (qPCR): This technique measures the amount of DNA as it is amplified, allowing for the quantification of DNA or RNA in a sample. It is widely used for gene expression studies. Digital PCR (dPCR): This method provides absolute quantification of DNA molecules, making it highly sensitive for detecting low-abundance mutations and rare genetic events. Reverse Transcription PCR (RT-PCR): This technique converts RNA into DNA before amplification, enabling the study of gene expression and viral RNA in cancer research.
