What are the Limitations of PCR in Cancer Research?
Despite its advantages, PCR has some limitations:
Contamination Risk: PCR is highly sensitive, making it susceptible to contamination, which can lead to false-positive results. Quantification Challenges: While qPCR and dPCR provide quantification, conventional PCR does not, which can limit its utility in certain applications. Complexity: Designing specific primers and optimizing PCR conditions can be complex and time-consuming. Cost: Advanced PCR techniques, such as dPCR, can be expensive, limiting their accessibility in some settings.
