Several factors influence the depth and effectiveness of light penetration in tissues:
1. Wavelength of Light: Different wavelengths penetrate tissues to varying extents. Generally, near-infrared (NIR) light (650-900 nm) offers deeper penetration compared to visible light. 2. Tissue Composition: The presence of melanin, hemoglobin, and water can absorb and scatter light, impacting penetration depth. 3. Tumor Location: Tumors located deeper within the body require more advanced techniques to ensure adequate light delivery.