The Yeast Two Hybrid (Y2H) screening is a molecular biology technique used to detect protein-protein interactions. Developed in the late 1980s, this method employs the yeast Saccharomyces cerevisiae as a living host to explore the interactions between two proteins, termed the "bait" and the "prey". When these proteins interact, they enable the expression of a reporter gene, providing a straightforward readout of the interaction.
