The Yeast Two Hybrid (Y2H) screening is a molecular biology technique used to discover protein–protein interactions (PPIs). By leveraging the simple eukaryotic system of yeast, scientists can identify and map interactions between various proteins. This technique employs a bait and prey system, where the protein of interest (bait) is fused to a DNA-binding domain, and a potential interacting protein (prey) is fused to a transcriptional activation domain. If the bait and prey proteins interact, a reporter gene is activated, signaling a successful interaction.
