What Techniques are Used to Measure mRNA Expression?
Several techniques are employed to measure mRNA expression levels in cancer research. These include:
1. Quantitative PCR (qPCR): This technique amplifies and quantifies specific mRNA sequences. 2. Microarrays: These allow for the simultaneous analysis of thousands of mRNA transcripts. 3. RNA sequencing (RNA-Seq): This provides a comprehensive view of the transcriptome, including the identification of novel transcripts and splice variants.
