What Technologies Are Used for Mutation Detection?
Several advanced technologies are employed for mutation detection, including:
Next-Generation Sequencing (NGS): This high-throughput method allows for the comprehensive analysis of multiple genes simultaneously, providing detailed information about genetic mutations. Polymerase Chain Reaction (PCR): PCR amplifies specific DNA sequences, making it easier to study mutations in those regions. Fluorescence In Situ Hybridization (FISH): This technique uses fluorescent probes to detect specific DNA sequences within the chromosomes, useful for identifying genetic abnormalities. Comparative Genomic Hybridization (CGH): CGH compares differences between normal and cancerous DNA, identifying regions of gain or loss in the genome. Single Nucleotide Polymorphism (SNP) Arrays: These arrays can detect single nucleotide changes, insertions, deletions, and copy number variations across the genome.
