Several methods are employed to authenticate biological samples in cancer research. These include:
DNA profiling: Using short tandem repeat (STR) analysis to verify the genetic identity of cell lines. Karyotyping: Examining the chromosomal composition to detect abnormalities and confirm species identity. Microscopy: Visual inspection of cells to identify morphological characteristics. Mycoplasma testing: Detecting contamination by mycoplasma, a common contaminant in cell cultures. Gene expression profiling: Comparing gene expression patterns to reference data to confirm cell type or tissue origin.
