Despite the potential of circRNAs in cancer research, there are several challenges: 1. Detection and Quantification: The closed loop structure of circRNAs makes it difficult to distinguish them from their linear counterparts using traditional RNA sequencing methods. Specialized techniques and bioinformatics tools are required. 2. Functional Validation: Demonstrating the functional roles of circRNAs in cancer requires extensive experimental validation, including loss-of-function and gain-of-function studies. 3. Clinical Translation: Moving from basic research to clinical applications involves rigorous testing to ensure the safety and efficacy of circRNA-based therapies.
