The procedures for performing cell-based assays generally follow a series of steps:
1. Cell Culture: Cancer cells are cultured in appropriate conditions to ensure their growth and viability. 2. Treatment: Cells are treated with the compounds under investigation, which could be drugs, small molecules, or other therapeutic agents. 3. Measurement: The effects of the treatment are measured using various detection methods, such as fluorescence, luminescence, or absorbance. 4. Analysis: Data obtained from the measurements are analyzed to determine the impact of the treatment on cancer cell behavior.
