Despite their advantages, cell-based assays face several challenges:
1. Complexity of Cancer: Cancer is a highly heterogeneous disease, and cell-based assays may not fully capture the complexity of human tumors. 2. Reproducibility: Variability in cell lines and experimental conditions can affect the reproducibility of results. 3. Microenvironment: The tumor microenvironment plays a critical role in cancer progression, and it can be difficult to replicate these conditions in vitro.
