Detecting genetic mutations in cancer involves several techniques:
Sequencing Technologies: Methods like next-generation sequencing (NGS) allow for comprehensive analysis of the cancer genome, identifying point mutations, indels, CNVs, and more. Polymerase Chain Reaction (PCR): PCR-based methods can amplify specific DNA regions to detect known mutations. Fluorescence In Situ Hybridization (FISH): FISH can identify chromosomal rearrangements and copy number changes by using fluorescent probes.
