histopathology slides

How are Histopathology Slides Prepared?

The preparation of histopathology slides involves several steps:
1. Tissue Fixation: The tissue sample is preserved using chemicals like formalin to prevent decay and maintain cellular structure.
2. Embedding: The fixed tissue is embedded in paraffin wax to create a solid block that can be easily cut into thin sections.
3. Sectioning: The paraffin block is sliced into very thin sections using a microtome.
4. Staining: The sections are stained with dyes, such as hematoxylin and eosin (H&E), to highlight different structures within the tissue.
5. Mounting: The stained sections are placed on glass slides and covered with a thin glass cover slip for microscopic examination.

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