The generation of a count matrix typically involves several steps: 1. Sequencing: Obtain RNA-Seq or DNA-Seq data from cancerous and non-cancerous samples. 2. Mapping: Align the sequencing reads to a reference genome. 3. Counting: Calculate the number of reads that map to each gene.
These steps involve various bioinformatics tools and pipelines, such as STAR, HISAT2, and HTSeq.
