How is CpG Methylation Analyzed in Cancer Research?
Several techniques are used to analyze CpG methylation in cancer research. These include bisulfite sequencing, which converts unmethylated cytosines to uracil, allowing for methylation mapping at single-nucleotide resolution. Other methods such as methylation-specific PCR and pyrosequencing are also commonly used to quantify methylation levels. High-throughput methods like Illumina Infinium MethylationEPIC BeadChip enable genome-wide methylation profiling.
