EMT can be detected and measured using a variety of techniques. One common method is the analysis of biomarkers associated with epithelial and mesenchymal states. For instance, the loss of epithelial markers such as E-cadherin and the gain of mesenchymal markers like N-cadherin and vimentin are indicative of EMT. Techniques such as immunohistochemistry, Western blotting, and quantitative PCR are commonly used to assess these changes.
