Exome sequencing involves several steps: 1. DNA Extraction: DNA is extracted from a patient's tissue sample, often from blood or tumor tissue. 2. Library Preparation: The extracted DNA is fragmented and adapters are added to create a library of DNA fragments. 3. Enrichment: The library is enriched for exonic regions using probes that capture the exome sequences. 4. Sequencing: The enriched library is sequenced using high-throughput sequencing technologies. 5. Data Analysis: Bioinformatics tools are used to align the sequences to a reference genome and identify mutations.
