The process typically involves obtaining a tissue sample through a biopsy or surgical resection. The sample is then fixed, embedded in paraffin, and sectioned onto slides. These slides are treated with antibodies specific to the Ki-67 protein. The binding of these antibodies is visualized using various detection systems, such as chromogenic or fluorescent labels. The stained slides are examined under a microscope, and the percentage of Ki-67-positive cells is calculated.
