The interpretation of qRT-PCR data involves several steps:
Threshold Cycle (Ct) Value: The Ct value is the cycle number at which the fluorescence signal exceeds the background level. Lower Ct values indicate higher initial quantities of the target gene. Standard Curves: Standard curves generated from known concentrations of target DNA are used to quantify unknown samples. Relative Quantification: The ΔΔCt method is commonly used for relative quantification, comparing the expression levels of the target gene to a reference gene and a control sample.
