What Are Some Recent Advances in 2D Monolayer Cultures?
Recent advances aim to improve the relevance and predictive power of 2D monolayer cultures. Some approaches include: 1. Co-Culture Systems: Growing cancer cells alongside other cell types, such as stromal or immune cells, to better mimic the tumor microenvironment. 2. Microfluidics: Incorporating microfluidic devices to create dynamic culture conditions, allowing for more precise control over nutrient and oxygen supply. 3. Biomimetic Surfaces: Using surfaces coated with extracellular matrix proteins or other biomaterials to enhance cell-matrix interactions and more closely mimic in vivo conditions.
