Comprehensive Analysis: mIHC allows for the simultaneous examination of multiple biomarkers, providing a more holistic view of the tumor microenvironment. Spatial Context: This technique maintains the spatial context of the tissue, which is essential for understanding cell interactions and tumor architecture. Enhanced Sensitivity: The use of multiple fluorophores increases the sensitivity and specificity of detection, allowing for the identification of low-abundance proteins. Data Integration: mIHC data can be integrated with other types of data, such as genomic or transcriptomic data, to provide a multi-dimensional view of the tumor.