Despite its advantages, western blotting has some limitations:
Time-Consuming: The process can be labor-intensive and time-consuming. Quantitative Limitations: While it can be quantitative, it is less precise than some other methods. Antibody Dependency: The quality of results depends on the specificity and affinity of the antibodies used. Technical Variability: Variability can occur due to differences in sample preparation, gel electrophoresis, and transfer efficiency.
