How to Interpret Western Blot Results in Cancer Research?
Interpreting western blot results involves analyzing the presence, absence, and intensity of protein bands:
Band Intensity: Indicates the relative amount of the target protein. Band Size: Confirms the molecular weight of the protein, ensuring specificity. Control Proteins: Use of loading controls (e.g., GAPDH, β-actin) ensures equal sample loading and transfer efficiency. Normalization: Quantify target proteins relative to control proteins for accurate comparison.
