What are the Challenges of Co-Immunoprecipitation?
- Non-Specific Binding: One of the biggest challenges is non-specific binding of proteins to the antibody or beads, which can lead to false positives. - Epitope Masking: The epitope recognized by the antibody may be masked by interacting proteins, preventing efficient precipitation. - Antibody Quality: The success of Co-IP heavily relies on the quality and specificity of the antibody used.
