What are the Key Steps in a Co-Immunoprecipitation Experiment?
1. Cell Lysis: Cells are lysed to release their protein contents. 2. Incubation with Antibody: An antibody specific to the bait protein is added to the lysate. 3. Precipitation: The antibody-protein complex is precipitated using Protein A/G beads. 4. Washing: The beads are washed to remove non-specifically bound proteins. 5. Elution and Analysis: The proteins are eluted from the beads and analyzed, often by Western blotting or mass spectrometry.
