Despite its advantages, in vivo screening also presents several challenges:
Ethical considerations: The use of live animals in research raises ethical questions, necessitating strict regulatory guidelines and ethical review processes. Cost and time: In vivo studies are often more expensive and time-consuming than in vitro studies. Complexity: The biological complexity of living organisms can introduce variability and complicate data interpretation. Translatability: Results from animal models do not always translate directly to human patients, necessitating further validation in clinical trials.
