Prospero exerts its effects through various mechanisms, such as regulating the expression of genes involved in cell cycle control, apoptosis, and cell migration. In some cancers, Prox1 enhances the epithelial-to-mesenchymal transition (EMT), a process that allows cancer cells to gain migratory and invasive properties. Conversely, in other contexts, it can inhibit EMT and promote a more differentiated, less aggressive state.