What are the Methods to Detect Nonsense Mutations?
Detecting nonsense mutations involves several techniques. Polymerase Chain Reaction (PCR) followed by DNA sequencing is a common approach. Next-generation sequencing (NGS) allows for the comprehensive analysis of multiple genes simultaneously, providing a detailed mutation profile. Sanger sequencing is also used for targeted mutation detection. Additionally, RNA sequencing can identify nonsense mutations by evaluating the expressed RNA transcripts.
