The development of a robust assay involves several key steps:
1. Identifying the Target: Determine the specific biomarker, cell type, or pathway to be analyzed. 2. Selection of Assay Format: Choose the appropriate type of assay based on the target and the desired outcome. 3. Optimization: Fine-tune assay conditions to achieve the best sensitivity, specificity, and reproducibility. 4. Validation: Test the assay using known standards and controls to ensure accuracy and reliability. 5. Implementation: Apply the assay in a clinical or research setting and continually monitor its performance.
