What Techniques are Used in Immune Landscape Mapping?
Several advanced techniques are employed in immune landscape mapping, including:
Flow Cytometry and Mass Cytometry (CyTOF) – These techniques allow for the detailed phenotyping of immune cells using multiple markers simultaneously. Single-Cell RNA Sequencing (scRNA-seq) – This method provides insights into the gene expression profiles of individual immune cells within the tumor. Immunohistochemistry (IHC) and Immunofluorescence – These techniques enable visualization and quantification of immune cells in tissue sections. Multiplex Imaging – Techniques like MIBI and IMC allow for the spatial mapping of multiple immune markers simultaneously.
