Several technologies are employed for the detection of fusion genes. RNA sequencing (RNA-seq) is a powerful tool that provides insights into the transcriptome, allowing for the detection of gene fusions at the RNA level. Paired-end sequencing and long-read sequencing technologies like PacBio or Oxford Nanopore offer advantages in identifying complex fusion events. Single-cell sequencing can also be utilized to detect fusion genes in individual cancer cells, providing a deeper understanding of tumor heterogeneity.
