Detecting cancer variants involves various techniques, including:
1. Next-Generation Sequencing (NGS): This technology allows for comprehensive analysis of the cancer genome, identifying both common and rare variants.
2. Polymerase Chain Reaction (PCR): PCR is a technique used to amplify specific DNA sequences, making it easier to identify known mutations.
3. Fluorescence In Situ Hybridization (FISH): FISH is used to detect structural changes in chromosomes, such as translocations and amplifications.
4. Immunohistochemistry (IHC): IHC involves staining tissues with antibodies to detect the presence of specific proteins that may indicate genetic changes.
