Isolating and characterizing EVs involves several techniques:
1. Ultracentrifugation: A common method where differential centrifugation steps are used to separate EVs based on size and density. 2. Size Exclusion Chromatography: Separates EVs from other components based on size. 3. Immunoaffinity Capture: Utilizes antibodies against specific EV surface markers for isolation. 4. Characterization: Techniques like Western blotting, flow cytometry, and electron microscopy are used to analyze the size, morphology, and content of EVs.
