How Are Protein Interactions Studied in Cancer Research?
Several techniques are employed to study protein interactions in cancer:
Co-immunoprecipitation (Co-IP): This method isolates protein complexes from cell lysates using specific antibodies. It helps identify potential interacting partners of a protein of interest. Yeast Two-Hybrid Screening: This genetic technique identifies protein-protein interactions by detecting the physical interaction between two proteins in yeast cells. Mass Spectrometry: Used to analyze protein complexes and identify their constituent proteins. It provides a comprehensive view of the protein interaction network. Fluorescence Resonance Energy Transfer (FRET): A technique that monitors interactions between proteins in living cells, based on energy transfer between fluorophores attached to the proteins.
