The process of ICC involves several steps: 1. Sample Preparation: Cells are fixed to preserve their structure and attached to a slide. 2. Antibody Binding: Primary antibodies specific to the target antigen are applied to the sample. These antibodies bind to the antigen of interest. 3. Detection: Secondary antibodies, which are conjugated to a detectable marker (such as a fluorescent dye or an enzyme), are introduced. These bind to the primary antibodies. 4. Visualization: The marker on the secondary antibodies allows for the visualization of the antigen under a microscope.
