- Antibody Quality: The success of ICC relies heavily on the quality and specificity of the antibodies used. Poor-quality antibodies can lead to non-specific binding and inaccurate results. - Technical Complexity: ICC requires careful optimization of experimental conditions, including fixation methods, antibody concentrations, and detection techniques. - Interpretation: The interpretation of ICC results can be subjective and requires expertise, particularly in differentiating between specific and non-specific staining.
