fluorescent in situ hybridization

How is FISH Performed?

The FISH process involves several key steps:
Sample Preparation: Cells or tissue samples are prepared and fixed on a microscope slide.
Probe Hybridization: Fluorescently labeled DNA probes are applied to the sample and allowed to hybridize to their complementary DNA sequences.
Washing: Excess probes are washed away, leaving only the probes that have bound to their target sequences.
Visualization: The sample is examined under a fluorescence microscope, and the fluorescent signals are analyzed to identify genetic abnormalities.

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