Formulate Hypotheses: Establish a null hypothesis (no difference) and an alternative hypothesis (there is a difference). Collect Data: Gather the necessary data from clinical trials, patient records, or laboratory experiments. Calculate the T-Statistic: Use the mean, variance, and sample size to compute the T-statistic. Compare to Critical Value: Compare the T-statistic to a critical value from the T-distribution table based on the desired significance level (e.g., 0.05). Interpret Results: If the T-statistic exceeds the critical value, reject the null hypothesis in favor of the alternative hypothesis.