Several advanced variants of ChIP have been developed to address some of its limitations and enhance its capabilities:
- ChIP-seq: Combines ChIP with high-throughput sequencing to provide a genome-wide map of protein-DNA interactions. - ChIP-exo: Utilizes exonuclease digestion to provide higher resolution mapping of protein binding sites. - CUT&RUN: A newer technique that uses a fusion protein to cleave DNA at protein binding sites, offering an alternative to traditional ChIP with reduced background noise.
