While organoids offer many advantages, they also have limitations:
Complexity: Creating and maintaining organoids is technically challenging and requires specialized expertise. Standardization: There is a lack of standardized protocols for generating and using organoids, which can lead to variability in results. Lack of Immune Cells: Most organoid models do not include immune cells, which play a crucial role in cancer progression and response to therapy. Scalability: Large-scale production of organoids is still a challenge, limiting their use in high-throughput applications.
